This study evaluates the use of automatic classification using AI on eight closely related radiolarian species of the genus Podocyrtis based on MobileNet CNN. Species belonging to Podocyrtis are useful for middle Eocene biostratigraphy. Numerous images of Podocyrtis species from the tropical Atlantic Ocean were used to train and validate the CNN. An overall accuracy of about 91 % was obtained. Additional Podocyrtis specimens from other ocean realms were used to test the predictive model.